Streamlined process development using the Micro24 Bioreactor system

Introduction The Pall Micro24 Bioreactor system is one of several microbioreactor systems that have been commercialised in recent years in response to the demand to reduce costs and shorten process development time lines. We have previously demonstrated that the Micro24 Bioreactor system can be integrated successfully into the later stages of cell line screening programmes and that the results correlate well with those from more conventional methods [1]. Further process development for these selected cell lines traditionally utilises bench top bioreactors to define appropriate process conditions giving the desired process outcomes although this approach can be time consuming and resource intensive. However the Micro24 Bioreactor system allows up to 24 different process conditions to be run concurrently thereby facilitating efficient process development. This work describes the use of the Micro24 Bioreactor system to identify improved process conditions for different cell lines and their subsequent validation in bioreactors.